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These landmark studies showed that mice with adipose-tissue-specific NNMT knockout remained lean when fed high-fat diets that caused wild-type mice to become obese, and that these protected mice had increased energy expenditure, enhanced fatty acid oxidation, improved glucose tolerance, and elevated NAD+ levels in adipose tissue

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After the washing step the R6G dye of the internalized non-targeted or targeted nanocarriers in living hECs was imaged using the 543 nm laser line on a Leica TCS SP5 confocal laser scanning microscope equipped with a sample holder kept at 37 C

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